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You are here: The Britz-McKibbin Laboratory > Publications > High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis.

G Segui-Lines, J MA Gavina, J C D'Amaral, and P Britz-McKibbin (2007)

High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis.

Analyst, 132(8):741-4.

Dynamic ligand exchange-affinity capillary electrophoresis (DLE-ACE) is introduced as a convenient platform for assessing the conformational stability and relative affinity of a holoprotein to different ligands without off-line sample pretreatment, since ligand exchange and protein unfolding processes are integrated in-capillary during electromigration.

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